Endocrine Abstracts

Background: In our previous works the interdependence of DNA demethylation with proliferation and differentiation of pituitary neuroendocrine tumours (PitNET) and the inhibitory effect of Aspirin on pituitary cell proliferation were demonstrated. Although the role of Aspirin in epigenetic regulation was described in other malignancies, its correlation with pituitary tumorigenesis is currently unknown.Our objective: was to investigate the genomic and epig...

Introduction: Spindle cell oncocytomas (SCO) of the pituitary are rare tumors accounting for 0.1–0.4% of all sellar tumors. Due to its rarity, little information is available regarding its pathogonesis. The altered gene expression and the possible pathogenetic role of microRNAs (miRNAs) have been identified in many tumor types, however, until now ther is no data regarding their role in pituitary oncocytoma.Materials and methods: Total RNA was extrac...

Introduction: In vitro monolayer cell cultures are not able to faithfully model the biological features of the three dimensional (3D) structure of solid tumours. There are limited information about the applicability of 3D cell culturing methods for modeling of tumours of endocrine organs.Aim: The aim of our work was the establishment and characterization of three-dimensional in vitro models of various endocrine tumour cells in order to ...

Pheochromocytoma/paragangliomas (Pheo/PGL) are rare neuroendocrine cancers with strong genetic background. Mutations in the SDHB subunit of succinate dehydrogenase (SDH) predispose to malignant disease with limited therapeutic options and poor prognosis. Novel prognostic markers and therapeutic targets are required to decrease the high mortality rate. Glutaminases play a crucial role in the metabolism of SDH impaired tumor cells. By using a host of cellular and molecu...